Sulfate Auto-Test™ The Orion AQUAfast IV Auto-Test kit is intended for use with the Orion AQ4000 Advanced Colorimeter. For detailed setup and measurement procedures for the AQ4000, consult your colorimeter manual. NOTE: Before testing, zero the Orion AQ4000 using a sealed zero vial from the Orion AQUAfast IV Zero Auto-Test kit, AQ4ZER. If the sample is colored, dilute the sample in two steps. Dilute 5 mL of sample to 15 mL with deionized water, using the sample cup provided with the Auto-Test kit. Transfer the diluted sample to a clean, dry beaker or vial. Then, dilute 5 mL of this diluted sample to 20 mL with deionized water, using the sample cup. Put this twice-diluted sample into a zero cuvette with removeable cap, and zero the meter. See the olorimeter manual for details on the Zero Procedure for the Orion AQ4000. Safety Information Read MSDS before performing this test procedure. Wear safety glasses and gloves. Sample Pretreatment If the sample is turbid, it must be filtered prior to performing this test procedure. Test Procedure 1. Fill the sample cup to the 25 mL mark with the Place a column on the tip of the Auto-Test cuvette so that it fits snugly (fig 2). Place the tapered tip of the cuvette/column assembly into the sample cup in the small space formed by the plastic chip adhered to the side of the cup. Snap the tip of the cuvette by simply pressing down on the cuvette. The cuvette will fill leaving a small bubble to facilitate mixing. Do not remove the cuvette from the cup until filling is complete. 4. Mix the contents of the cuvette by inverting it several times, allowing the bubble to travel from end to end each time. Wipe all liquid from the NOTE: If the column becomes loose during handling, after the filling process, it may be 5. Insert cuvette into AQ4000. Align the T on the Auto-Test cuvette with the ♦ on the adapter to obtain a continuous beeping and view ******* across the display. If ******* and beeping is not observed, rotate cuvette right or left to initiate the measurement. 6. Immediately cover the cuvette with the cuvette cover. 7. The AQ4000 will be begin a 1 minute countdown. After the countdown is completed, the AQ4000 will automatically proceed to the measure mode. 8. Record the concentration reading from the S04 or log measurement into the data logger Test Method The Sulfate Auto-Test method employs the barium sulfate method.1-2 Sulfate ion reacts with barium chloride in an acidic solution to form a suspension of barium sulfate crystals of uniform size. The resulting turbidity is proportional to the sulfate concentration of the sample. Results are expressed 1. Standard Methods for the Treatment of Water and Wastewater, 20th Edition, 4B00-SO42-E. Turbidimetrio Method, pp 4-178. 2. EPA Approved Method 375.4 for Sulfate (Turbidimetrio) (1978). Ordering Information Orion Description AQ4ZER AQUAfast IV Zero Vial AQ4000 AQUAfast IV Advanced Colorimeter Environmental Instruments Water Analysis For updated contact information, visit www.thermo.com North America 12-16 Sedgeway Business Park Witchford, Cambridgeshire Wanchai, Hong Kong Customer Support e-mail: [email protected] Analyze • Detect • Measure • Control™ ELECTRON CORPORATION